Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model

0438976 - ÚFCH JH 2015 RIV CZ eng J - Článek v odborném periodiku
Zídková, J. - Melčová, M. - Bartošová, K. - Šestáková, Ivana - Zídek, Václav - Száková, J. - Miholová, D. - Tlustoš, P.
Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model.
Czech Journal of Animal Science. Roč. 59, č. 12 (2014), s. 548-556. ISSN 1212-1819. E-ISSN 1805-9309
Grant CEP: GA AV ČR IAA400400806; GA ČR GA13-04580S
Institucionální podpora: RVO:61388955 ; RVO:67985823
Klíčová slova: metal intoxication * liver * blood plasma
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 1.183, rok: 2014

The response of different strains of laboratory rats (Rattus norvegicus L.) on both acute (via intraperitoneal injection) and chronic (via drinking water and/or diet) cadmium intoxication was investigated in the model study. The rat strains Long Evans (LE), Spontaneously hypertensive rat (SHR), and Brown Norway (BN) were tested and compared, and total Cd levels and metallothionein (MT) concentrations were determined in the liver of experimental animals. The liver MT concentrations were determined by using adsorptive chronopotentiometry and modified Brdicka reaction and were significantly correlated (r = 0.965) with the total liver Cd content. Moreover, the Cd application resulted in increasing zinc liver contents confirming intensive MT synthesis in the rat liver. In the blood plasma, specific enzymatic activity of glutathione reductase (GR) and glutathione-S-transferase (GST) was determined suggesting increasing activity of GR with the amount of applied Cd for all three strains, whereas ambiguous results have been found for the activity of GST. Therefore, MT concentrations seemed to be more sensitive indicators of the Cd intoxication compared to the assessment of the specific enzymatic activity.
Trvalý link: http://hdl.handle.net/11104/0242316