Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup

0444547 - BC 2016 RIV CH eng J - Článek v odborném periodiku
Wolters, B. - Kyselková, Martina - Krögerrecklenfort, E. - Kreuzig, R. - Smalla, K.
Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup.
Frontiers in Microbiology. Roč. 5, January (2015), Article 765. ISSN 1664-302X. E-ISSN 1664-302X
Grant CEP: GA MŠMT(CZ) EE2.3.30.0032
Institucionální podpora: RVO:60077344
Klíčová slova: IncP-1 epsilon plasmid * class 1 integrons * biogas plant digestate * antibiotic resistance * exogenous plasmid isolation
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 4.165, rok: 2015

Manure is known to contain residues of antibiotics administered to farm animals as well as bacteria carrying antibiotic resistance genes (ARGs). These genes are often located on mobile genetic elements. In biogas plants (BGPs), organic substrates such as manure and plant material are mixed and fermented in order to provide energy, and resulting digestates are used for soil fertilization. The fate of plasmid carrying bacteria from manure during the fermentation process is unknown. The present study focused on transferable antibiotic resistance plasmids from digestates of seven BGPs, using manure as a co-substrate, and their phenotypic and genotypic characterization. Plasmids conferring resistance to either tetracycline or sulfadiazine were captured by means of exogenous plasmid isolation from digestates into Pseudomonas putida KT2442 and Escherichia coli CV601 recipients, at transfer frequencies ranging from 10(-5) to 10(-7). Transconjugants (n = 101) were screened by PCR-Southern blot hybridization and real-time PCR for the presence of IncP-1, IncP-1 epsilon, IncW, IncN, IncP-7, IncP-9, LowGC, and IncQ plasmids. While 61 plasmids remained unassigned, 40 plasmids belonged to the IncP-1 epsilon subgroup. All these IncP-1 epsilon plasmids were shown to harbor the genes tet(A), sul1, qacE Delta 1, intl1, and integron gene cassette amplicons of different size. Further analysis of 16 representative IncP-1 epsilon plasmids showed that they conferred six different multiple antibiotic resistance patterns and their diversity seemed to be driven by the gene cassette arrays. IncP-1 epsilon plasmids displaying similar restriction and antibiotic resistance patterns were captured from different BGPs, suggesting that they may be typical of this environment.
Trvalý link: http://hdl.handle.net/11104/0249919