Počet záznamů: 1  

Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids

  1. 1.
    0449774 - FGÚ 2016 RIV GR eng J - Článek v odborném periodiku
    Olejár, Tomáš - Pajuelo-Reguera, David - Alán, Lukáš - Dlasková, Andrea - Ježek, Petr
    Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids.
    Molecular Medicine Reports. Roč. 12, č. 4 (2015), s. 5185-5190. ISSN 1791-2997. E-ISSN 1791-3004
    Grant CEP: GA ČR(CZ) GAP302/10/0346; GA MŠk(CZ) EE2.3.30.0025
    Institucionální podpora: RVO:67985823
    Klíčová slova: mitochondrial nucleoid * single-stranded DNA-binding protein * photoconvertible fluorescent protein Eos
    Kód oboru RIV: EA - Morfologické obory a cytologie
    Impakt faktor: 1.559, rok: 2015

    A small limited number of large aggregates of mtSSB EosFP tagged protein started to form in the cells, which exhibited a great co localization with the noted coupled positions. Antibody staining and 3D immunocytochemistry confirmed that nucleoid components such as TFAM and DNA were co localized with these aggregates. Furthermore, the observed reduction of the mtDNA copy number in mtSSB EosFP transfected cells suggested a possible impairment of nucleoid function. In conclusion, the present study demonstrated that coupled nucleoids are synchronized by mtSSB EosFP overexpression and visualized through their equal binding capacity to mtSSB EosFP tagged protein. This observation suggested parallel replication and transcription activity of nucleoid couples native from a parental one. Preserved coupling in late stages of artificial EosFP mediated aggregation of tagged proteins suggested a rational manner of mitochondrial branching that may be cell-type specifically dependent on hierarchical nucleoid replication
    Trvalý link: http://hdl.handle.net/11104/0251977

     
     
Počet záznamů: 1  

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