Počet záznamů: 1
Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids
- 1.0449774 - FGÚ 2016 RIV GR eng J - Článek v odborném periodiku
Olejár, Tomáš - Pajuelo-Reguera, David - Alán, Lukáš - Dlasková, Andrea - Ježek, Petr
Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids.
Molecular Medicine Reports. Roč. 12, č. 4 (2015), s. 5185-5190. ISSN 1791-2997. E-ISSN 1791-3004
Grant CEP: GA ČR(CZ) GAP302/10/0346; GA MŠk(CZ) EE2.3.30.0025
Institucionální podpora: RVO:67985823
Klíčová slova: mitochondrial nucleoid * single-stranded DNA-binding protein * photoconvertible fluorescent protein Eos
Kód oboru RIV: EA - Morfologické obory a cytologie
Impakt faktor: 1.559, rok: 2015
A small limited number of large aggregates of mtSSB EosFP tagged protein started to form in the cells, which exhibited a great co localization with the noted coupled positions. Antibody staining and 3D immunocytochemistry confirmed that nucleoid components such as TFAM and DNA were co localized with these aggregates. Furthermore, the observed reduction of the mtDNA copy number in mtSSB EosFP transfected cells suggested a possible impairment of nucleoid function. In conclusion, the present study demonstrated that coupled nucleoids are synchronized by mtSSB EosFP overexpression and visualized through their equal binding capacity to mtSSB EosFP tagged protein. This observation suggested parallel replication and transcription activity of nucleoid couples native from a parental one. Preserved coupling in late stages of artificial EosFP mediated aggregation of tagged proteins suggested a rational manner of mitochondrial branching that may be cell-type specifically dependent on hierarchical nucleoid replication
Trvalý link: http://hdl.handle.net/11104/0251977
Počet záznamů: 1