Serological Markers of Enterocyte Damage and Apoptosis in Patients With Celiac Disease, Autoimmune Diabetes Mellitus and Diabetes Mellitus Type 2

0452699 - MBÚ 2016 RIV CZ eng J - Článek v odborném periodiku
Hoffmanová, I. - Sánchez, Daniel - Hábová, Věra - Anděl, M. - Tučková, Ludmila - Tlaskalová-Hogenová, Helena
Serological Markers of Enterocyte Damage and Apoptosis in Patients With Celiac Disease, Autoimmune Diabetes Mellitus and Diabetes Mellitus Type 2.
Physiological Research. Roč. 64, č. 4 (2015), s. 537-546. ISSN 0862-8408. E-ISSN 1802-9973
Grant CEP: GA ČR GA13-14608S; GA ČR(CZ) GAP304/11/1252; GA MZd(CZ) NT13483; GA TA ČR TA01010737; GA TA ČR TA04010762
Institucionální podpora: RVO:61388971
Klíčová slova: Cytokeratin 18 caspase-cleaved fragment * Intestinal fatty acid-binding protein * Soluble CD14
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 1.643, rok: 2015

Impairment of mucosal barrier integrity of small intestine might be causative in immune-mediated gastrointestinal diseases. We tested the markers of epithelial apoptosis - cytokeratin 18 caspase-cleaved fragment (cCK-18), and enterocyte damage - intestinal fatty acid-binding protein (I-FABP) and soluble CD14 (sCD14) in sera of patients with untreated celiac disease (CLD), those on gluten-free diet (CLD-GFD), patients with autoimmune diabetes mellitus (T1D), T1D with insulitis (T1D/INS), and diabetes mellitus type 2 (T2D). We found elevated levels of cCK-18 (P<0.001), I-FABP (P<0.01) and sCD14 (P<0.05) in CLD when compared to healthy controls. However, the levels of cCK-18 (P<0.01) and I-FABP (P<0.01) in CLD-GFD were higher when compared with controls. Interestingly, elevated levels of cCK-18 and I-FABP were found in T2D and T1D (P<0.001), and T1D/INS (P<0.01, P<0.001). Twenty-two out of 43 CLD patients were seropositive for cCK-18, 19/43 for I-FABP and 11/43 for sCD14; 9/30 of T2D patients were positive for cCK-18 and 5/20 of T1D/INS for sCD14, while in controls only 3/41 were positive for cCK-18, 3/41 for I-FABP and 1/41 for sCD14. We documented for the first time seropositivity for sCD14 in CLD and potential usefulness of serum cCK-18 and I-FABP as markers of gut damage in CLD, CLD-GFD, and diabetes
Trvalý link: http://hdl.handle.net/11104/0253614