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Novel X-Linked Genes Revealed by Quantitative Polymerase Chain Reaction in the Green Anole, Anolis carolinensis

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    0454370 - ÚŽFG 2016 RIV US eng J - Článek v odborném periodiku
    Rovatsos, M. - Altmanová, M. - Johnson Pokorná, Martina - Kratochvíl, L.
    Novel X-Linked Genes Revealed by Quantitative Polymerase Chain Reaction in the Green Anole, Anolis carolinensis.
    G3-Genes, Genomes, Genetics. Roč. 4, č. 11 (2014), s. 2107-2113. ISSN 2160-1836. E-ISSN 2160-1836
    Grant CEP: GA ČR GAP506/10/0718
    Institucionální podpora: RVO:67985904
    Klíčová slova: gene dosage * lizard * qPCR
    Kód oboru RIV: EG - Zoologie
    Impakt faktor: 3.198, rok: 2014

    The green anole, Anolis carolinensis (ACA), is the model reptile for a vast array of biological disciplines. It was the first nonavian reptile to have its genome fully sequenced. During the genome project, the XX/XY system of sex chromosomes homologous to chicken chromosome 15 (GGA15) was revealed, and 106 X-linked genes were identified. We selected 38 genes located on eight scaffolds in ACA and having orthologs located on GGA15, then tested their linkage to ACA X chromosome by using comparative quantitative fluorescent real-time polymerase chain reaction applied to male and female genomic DNA. All tested genes appeared to be X-specific and not present on the Y chromosome. Assuming that all genes located on these scaffolds should be localized to the ACA X chromosome, we more than doubled the number of known X-linked genes in ACA, from 106 to 250. While demonstrating that the gene content of chromosome X in ACA and GGA15 is largely conserved, we nevertheless showed that numerous interchromosomal rearrangements had occurred since the splitting of the chicken and anole evolutionary lineages. The presence of many ACA X-specific genes localized to distinct contigs indicates that the ACA Y chromosome should be highly degenerated, having lost a large amount of its original gene content during evolution. The identification of novel genes linked to the X chromosome and absent on the Y chromosome in the model lizard species contributes to ongoing research as to the evolution of sex determination in reptiles and provides important information for future comparative and functional genomics.
    Trvalý link: http://hdl.handle.net/11104/0255158

     
     
Počet záznamů: 1  

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