Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents.

0457446 - BTÚ 2016 RIV US eng J - Článek v odborném periodiku
Klučková, Katarína - Dong, L. F. - Bajziková, Martina - Rohlena, Jakub - Neužil, Jiří
Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents.
Methods in molecular biology. Roč. 1265, 07 Oct 2015 (2015), s. 181-194. ISSN 1940-6029
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: Animals * Antineoplastic Agents * drug effects * *pharmacology
Kód oboru RIV: EB - Genetika a molekulární biologie

Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue.
Trvalý link: http://hdl.handle.net/11104/0257845