Počet záznamů: 1
Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase
- 1.0457672 - BFÚ 2016 RIV GB eng J - Článek v odborném periodiku
Fajkus, Petr - Peška, Vratislav - Sitová, Z. - Fulnečková, Jana - Dvořáčková, Martina - Gogola, M. - Sýkorová, Eva - Hapala, J. - Fajkus, J.
Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase.
Plant Journal. Roč. 85, č. 3 (2016), s. 337-347. ISSN 0960-7412. E-ISSN 1365-313X
Grant CEP: GA ČR(CZ) GP13-10948P
Institucionální podpora: RVO:68081707
Klíčová slova: Allium * unusual telomere * telomerase
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 5.901, rok: 2016
Phylogenetic divergence in Asparagales plants is associated with switches in telomere sequences. The last switch occurred with divergence of the genus Allium (Amaryllidaceae) from the other Allioideae (formerly Alliaceae) genera, resulting in uncharacterized telomeres maintained by an unknown mechanism. To characterize the unknown Allium telomeres, we applied a combination of bioinformatic processing of transcriptomic and genomic data with standard approaches in telomere biology such as BAL31 sensitivity tests, terminal restriction fragment analysis, the telomere repeat amplification protocol (TRAP), and fluorescence insitu hybridization (FISH). Using these methods, we characterize the unusual telomeric sequence (CTCGGTTATGGG)(n) present in Allium species, demonstrate its synthesis by telomerase, and characterize the telomerase reverse transcriptase (TERT) subunit of Allium cepa.
Trvalý link: http://hdl.handle.net/11104/0258047
Počet záznamů: 1