Počet záznamů: 1
Potassium Uptake Mediated by Trk1 Is Crucial for Candida glabrata Growth and Fitness
- 1.0459360 - FGÚ 2017 RIV US eng J - Článek v odborném periodiku
Llopis-Torregrosa, Vincent - Hušeková, Barbora - Sychrová, Hana
Potassium Uptake Mediated by Trk1 Is Crucial for Candida glabrata Growth and Fitness.
PLoS ONE. Roč. 11, č. 4 (2016), e0153374. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR(CZ) GAP302/12/1151; GA ČR(CZ) GA16-03398S
GRANT EU: European Commission(XE) 606786 - ImResFun
Institucionální podpora: RVO:67985823
Klíčová slova: Candida glabrata * potassium uptake * Trk1 protein
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.806, rok: 2016
The maintenance of potassium homeostasis is crucial for all types of cells, including Candida glabrata. The C. glabrata genome contains only one gene encoding putative system for potassium uptake, the Trk1 uniporter. Therefore, its importance in maintaining adequate levels of intracellular potassium appears to be critical for C. glabrata cells. In this study, we first confirmed the potassium-uptake activity of the identified gene’s product by heterologous expression in a suitable S. cerevisiae mutant, further we generated a corresponding deletion mutant in C. glabrata and analysed its phenotype in detail. The obtained results show a pleiotropic effect on the cell physiology when CgTRK1 is deleted, affecting not only the ability of trk1delta to grow at low potassium concentrations, but also the tolerance to toxic alkali-metal cations and cationic drugs, as well as the membrane potential and intracellular pH. Taken together, our results find the sole potassium uptake system in C. glabrata cells to be a promising target in the search for its specific inhibitors and in developing new antifungal drugs.
Trvalý link: http://hdl.handle.net/11104/0259575
Počet záznamů: 1