ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling

0459971 - ÚEB 2017 RIV US eng J - Článek v odborném periodiku
Karampelias, M. - Neyt, P. - De Groeve, S. - Aesaert, S. - Coussens, G. - Rolčík, Jakub - Bruno, L. - De Winne, N. - Van Minnebruggen, A. - Van Montagu, M. - Ponce, M.R. - Micol, J.L. - Friml, J. - De Jaeger, G. - Van Lijsebettens, M.
ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 113, č. 10 (2016), s. 2768-2773. ISSN 0027-8424. E-ISSN 1091-6490
Institucionální podpora: RVO:61389030
Klíčová slova: Arabidopsis * PIN recycling * PP2A
Kód oboru RIV: EF - Botanika
Impakt faktor: 9.661, rok: 2016

The shaping of organs in plants depends on the intercellular flow of the phytohormone auxin, of which the directional signaling is determined by the polar subcellular localization of PIN-FORMED (PIN) auxin transport proteins. Phosphorylation dynamics of PIN proteins are affected by the protein phosphatase 2A (PP2A) and the PINOID kinase, which act antagonistically to mediate their apical-basal polar delivery. Here, we identified the ROTUNDA3 (RON3) protein as a regulator of the PP2A phosphatase activity in Arabidopsis thaliana. The RON3 gene was map-based cloned starting from the ron3-1 leaf mutant and found to be a unique, plant-specific gene coding for a protein with high and dispersed proline content. The ron3-1 and ron3-2 mutant phenotypes [i.e., reduced apical dominance, primary root length, lateral root emergence, and growth; increased ectopic stages II, IV, and V lateral root primordia; decreased auxin maxima in indole-3-acetic acid (IAA)-treated root apical meristems; hypergravitropic root growth and response; increased IAA levels in shoot apices; and reduced auxin accumulation in root meristems] support a role for RON3 in auxin biology. The affinity-purified PP2A complex with RON3 as bait suggested that RON3 might act in PIN transporter trafficking. Indeed, pharmacological interference with vesicle trafficking processes revealed that single ron3-2 and double ron3-2 rcn1 mutants have altered PIN polarity and endocytosis in specific cells. Our data indicate that RON3 contributes to auxin-mediated development by playing a role in PIN recycling and polarity establishment through regulation of the PP2A complex activity.
Trvalý link: http://hdl.handle.net/11104/0260116