Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications

0460075 - BC 2017 RIV NL eng J - Článek v odborném periodiku
Šafařík, Ivo - Štěpánek, M. - Uchman, M. - Šlouf, Miroslav - Baldíková, E. - Nýdlová, L. - Pospíšková, K. - Šafaříková, Miroslava
Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 67, October (2016), s. 486-492. ISSN 0928-4931. E-ISSN 1873-0191
Grant CEP: GA TA ČR(CZ) TE01020118; GA MŠMT(CZ) LO1507
Institucionální podpora: RVO:60077344 ; RVO:61389013
Klíčová slova: chitosan * immobilization * lipase * magnetic fluid * poly(methacrylic acid)
Kód oboru RIV: CD - Makromolekulární chemie; CD - Makromolekulární chemie (UMCH-V)
Impakt faktor: 4.164, rok: 2016

A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed. This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material. Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques. Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells. Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde. Baker's yeast cells (Saccharomyces cerevisiae./.) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
Trvalý link: http://hdl.handle.net/11104/0260232