Počet záznamů: 1
Physiology of Ca2+ signalling in stem cells of different origins and differentiation stages
- 1.0460352 - FGÚ 2017 RIV DE eng J - Článek v odborném periodiku
Forostyak, O. - Forostyak, S. - Kortus, Štěpán - Syková, E. - Verkhratsky, A. - Dayanithi, G.
Physiology of Ca2+ signalling in stem cells of different origins and differentiation stages.
Cell Calcium. Roč. 59, 2-3 (2016), s. 57-66. ISSN 0143-4160. E-ISSN 1532-1991
Grant CEP: GA ČR(CZ) GBP304/12/G069
Institucionální podpora: RVO:67985823
Klíčová slova: stem cells * Ca2+ channels * Ca2+ signalling * spontaneous Ca2+ oscillations
Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
Impakt faktor: 3.707, rok: 2016
Stem cells (SCs) of different origins have brought hope as potential tools for the treatment of neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and Amyotrophic Lateral Sclerosis. Calcium signalling plays a key role in SC differentiation and proliferation, and dysregulation of Ca2+ homeostasis may instigate pathological scenarios. Currently, the role of ion channels and receptors in SCs is not fully understood. In the recent years, we found that (i) the pre-differentiation of human embryonic SCs (hESCs) led to the activation of Ca2+ signalling cascades and enhanced the functional activities of these cells, (ii) the Ca2+ homeostasis and the physiological properties of hESC-derived neural precursors (NPs) changed during long term propagation in vitro, (iii) differentiation of NPs derived from human induced pluripotent SCs affects the expression of ion channels and receptors, (iv) these neuronal precursors exhibited spontaneous activity, indicating that their electrophysiological and Ca2+ handling properties are similar to those of mature neurones, and (v) in mesenchymal SCs isolated from the adipose tissue and bone marrow of rats the expression profile of ion channels and receptors depends not only on the differentiation conditions but also on the source from which the cells were isolated, indicating that the fate and functional properties of the differentiated cells are driven by intrinsic mechanisms. Together, identification and assignment of a unique ion channel and a Ca2+ handling footprint for each cell type would be necessary to qualify them as physiologically suitable for medical research, drug screening, and cell therapy.
Trvalý link: http://hdl.handle.net/11104/0260451
Počet záznamů: 1