Počet záznamů: 1  

Peripheral circadian clocks are diversely affected by adrenalectomy

  1. 1.
    0463099 - FGÚ 2017 RIV US eng J - Článek v odborném periodiku
    Soták, Matúš - Bryndová, Jana - Ergang, Peter - Vagnerová, Karla - Kvapilová, Pavlína - Vodička, Martin - Pácha, Jiří - Sumová, Alena
    Peripheral circadian clocks are diversely affected by adrenalectomy.
    Chronobiology International. Roč. 33, č. 5 (2016), s. 520-529. ISSN 0742-0528. E-ISSN 1525-6073
    Grant CEP: GA ČR(CZ) GA13-08304S
    Institucionální podpora: RVO:67985823
    Klíčová slova: adrenalectomy * circadian rhythms * corticosterone * peripheral clock
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 2.562, rok: 2016

    Glucocorticoids are considered to synchronize the rhythmicity of clock genes in peripheral tissues; however, the role of circadian variations of endogenous glucocorticoids is not well defined. In the present study, we examined whether peripheral circadian clocks were impaired by adrenalectomy. To achieve this, we tested the circadian rhythmicity of core clock genes (Bmal1, Per1-3, Cry1, RevErbα, Rora), clock-output genes (Dbp, E4bp4) and a glucocorticoid- and clock-controlled gene (Gilz) in liver, jejunum, kidney cortex, splenocytes and visceral adipose tissue (VAT). Adrenalectomy did not affect the phase of clock gene rhythms but distinctly modulated clock gene mRNA levels, and this effect was partially tissue-dependent. Adrenalectomy had a significant inhibitory effect on the level of Per1 mRNA in VAT, liver and jejunum, but not in kidney and splenocytes. Similarly, adrenalectomy down-regulated mRNA levels of Per2 in splenocytes and VAT, Per3 in jejunum, RevErbα in VAT and Dbp in VAT, kidney and splenocytes, whereas the mRNA amounts of Per1 and Per2 in kidney and Per3 in VAT and splenocytes were up-regulated. On the other hand, adrenalectomy had minimal effects on Rora and E4bp4 mRNAs. Adrenalectomy also resulted in decreased level of Gilz mRNA but did not alter the phase of its diurnal rhythm. Collectively, these findings suggest that adrenalectomy alters the mRNA levels of core clock genes and clock-output genes in peripheral organs and may cause tissue-specific modulations of their circadian profiles, which are reflected in changes of the amplitudes but not phases. Thus, the circulating corticosteroids are necessary for maintaining the high-amplitude rhythmicity of the peripheral clocks in a tissue-specific manner.
    Trvalý link: http://hdl.handle.net/11104/0262379

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.