Počet záznamů: 1  

Artificial proteins as allosteric modulators of PDZ3 and SH3 in two-domain constructs: A computational characterization of novel chimeric proteins

  1. 1.
    0463459 - FGÚ 2017 RIV US eng J - Článek v odborném periodiku
    Palani, K. - Pfeiferová, L. - Boušová, Kristýna - Bednárová, L. - Obšilová, Veronika - Vondrášek, J.
    Artificial proteins as allosteric modulators of PDZ3 and SH3 in two-domain constructs: A computational characterization of novel chimeric proteins.
    Proteins-Structure, Function and Bioinformatics. Roč. 84, č. 10 (2016), s. 1358-1374. ISSN 0887-3585. E-ISSN 1097-0134
    Institucionální podpora: RVO:67985823
    Klíčová slova: protein design * fusion proteins * PDZ3 * SH3 * Trp-cage * two domain proteins * molecular dynamics simulation * circular dichroism
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 2.289, rok: 2016

    Artificial multidomain proteins with enhanced structural and functional properties can be utilized in a broad spectrum of applications. The design of chimeric fusion proteins utilizing protein domains or one-domain miniproteins as building blocks is an important advancement for the creation of new biomolecules for biotechnology and medical applications. However, computational studies to describe in detail the dynamics and geometry properties of two-domain constructs made from structurally and functionally different proteins are lacking. Here, we tested an in silico design strategy using all-atom explicit solvent molecular dynamics simulations. The well-characterized PDZ3 and SH3 domains of human zonula occludens (ZO-1) (3TSZ), along with 5 artificial domains and 2 types of molecular linkers, were selected to construct chimeric two-domain molecules. The influence of the artificial domains on the structure and dynamics of the PDZ3 and SH3 domains was determined using a range of analyses. We conclude that the artificial domains can function as allosteric modulators of the PDZ3 and SH3 domains.
    Trvalý link: http://hdl.handle.net/11104/0262657

     
     
Počet záznamů: 1  

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