Decoding the complete arsenal for cellulose and hemicellulose deconstruction in the highly efficient cellulose decomposer Paenibacillus O199

0469004 - MBÚ 2017 RIV GB eng J - Článek v odborném periodiku
López-Mondejár, Rubén - Zühlke, D. - Větrovský, Tomáš - Becher, D. - Riedel, K. - Baldrian, Petr
Decoding the complete arsenal for cellulose and hemicellulose deconstruction in the highly efficient cellulose decomposer Paenibacillus O199.
Biotechnology for Biofuels. Roč. 9, MAY 14 (2016), s. 104. E-ISSN 1754-6834
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) EE2.3.30.0003; GA MŠMT(CZ) LM2015055; GA MŠMT(CZ) LD15086
Institucionální podpora: RVO:61388971
Klíčová slova: Cellulose * Hemicellulose * Paenibacillus
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 5.203, rok: 2016

Background: The search for new enzymes and microbial strains to degrade plant biomass is one of the most important strategies for improving the conversion processes in the production of environment-friendly chemicals and biofuels. In this study, we report a new Paenibacillus isolate, O199, which showed the highest efficiency for cellulose deconstruction in a screen of environmental isolates. Here, we provide a detailed description of the complex multicomponent O199 enzymatic system involved in the degradation of lignocellulose.

Results: We examined the genome and the proteome of O199 grown on complex lignocellulose (wheat straw) and on microcrystalline cellulose. The genome contained 476 genes with domains assigned to carbohydrate-active enzyme (CAZyme) families, including 100 genes coding for glycosyl hydrolases (GHs) putatively involved in cellulose and hemicellulose degradation. Moreover, 31 % of these CAZymes were expressed on cellulose and 29 % on wheat straw. Proteomic analyses also revealed a complex and complete set of enzymes for deconstruction of cellulose (at least 22 proteins, including 4 endocellulases, 2 exocellulases, 2 cellobiohydrolases and 2 beta-glucosidases) and hemicellulose (at least 28 proteins, including 5 endoxylanases, 1 beta-xylosidase, 2 xyloglucanases, 2 endomannanases, 2 licheninases and 1 endo-beta-1,3(4)-glucanase). Most of these proteins were secreted extracellularly and had numerous carbohydrate-binding domains (CBMs). In addition, O199 also secreted a high number of substrate-binding proteins (SBPs), including at least 42 proteins binding carbohydrates. Interestingly, both plant lignocellulose and crystalline cellulose triggered the production of a wide array of hydrolytic proteins, including cellulases, hemicellulases, and other GHs.


Trvalý link: http://hdl.handle.net/11104/0267377