Characterization of the Embryogenic Tissue of the Norway Spruce Including a Transition Layer between the Tissue and the Culture Medium by Magnetic Resonance Imaging

0471601 - ÚPT 2017 RIV SK eng J - Článek v odborném periodiku
Kořínek, Radim - Mikulka, J. - Hřib, J. - Hudec, Jiří - Havel, L. - Bartušek, Karel
Characterization of the Embryogenic Tissue of the Norway Spruce Including a Transition Layer between the Tissue and the Culture Medium by Magnetic Resonance Imaging.
Measurement Science Review. Roč. 17, č. 1 (2017), s. 19-26. ISSN 1335-8871. E-ISSN 1335-8871
Grant CEP: GA ČR(CZ) GA14-22777S; GA ČR GA13-09086S; GA MŠMT ED0017/01/01; GA MŠMT(CZ) LO1212
Institucionální podpora: RVO:68081731
Klíčová slova: Norway spruce * ESEs * mucilage * transition layer * MRI relaxometry
Obor OECD: Plant sciences, botany
Impakt faktor: 1.345, rok: 2017

The paper describes the visualization of the cells (ESEs) and mucilage (ECMSN) in an embryogenic tissue via magnetic resonance imaging (MRI) relaxometry measurement combined with the subsequent multi-parametric segmentation. The computed relaxometry maps T1 and T2 show a thin layer (transition layer) between the culture medium and the embryogenic tissue. The ESEs, mucilage, and transition layer differ in their relaxation times T1 and T2; thus, these times can be used to characterize the individual parts within the embryogenic tissue. The multi-parametric segmentation exploiting the T1 and T2 relaxation times as a classifier shows the distribution of the ESEs and mucilage within the embryogenic tissue. The discussed T1 and T2 indicators can be utilized to characterize both the growth-related changes in an embryogenic tissue and the effect of biotic/abiotic stresses, thus potentially becoming a distinctive indicator of the state of any examined embryogenic tissue.

Trvalý link: http://hdl.handle.net/11104/0268963