Identification and characterization of Xenopus tropicalis common progenitors of Sertoli and peritubular myoid cell lineages

0472500 - BTÚ 2017 RIV GB eng J - Článek v odborném periodiku
Tlapáková, T. - Nguyen, T.M.X. - Vegrichtova, M. - Šídová, Monika - Strnadova, K. - Bláhová, M. - Krylov, V.
Identification and characterization of Xenopus tropicalis common progenitors of Sertoli and peritubular myoid cell lineages.
Biology Open. Roč. 5, č. 9 (2016), s. 1275-1282. ISSN 2046-6390. E-ISSN 2046-6390
Grant CEP: GA MŠMT LK21305; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: Testicular somatic cells * Xenopus tropicalis * Migration potential
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.095, rok: 2016

The origin of somatic cell lineages during testicular development is controversial in mammals. Employing basal amphibian tetrapod Xenopus tropicalis we established a cell culture derived from testes of juvenile male. Expression analysis showed transcription of some pluripotency genes and Sertoli cell, peritubular myoid cell and mesenchymal cell markers. Transcription of germline-specific genes was downregulated. Immunocytochemistry revealed that a majority of cells express vimentin and co-express Sox9 and smooth muscle alpha-actin (Sma), indicating the existence of a common progenitor of Sertoli and peritubular myoid cell lineages. Microinjection of transgenic, red fluorescent protein (RFP)-positive somatic testicular cells into the peritoneal cavity of X. tropicalis tadpoles resulted in cell deposits in heart, pronephros and intestine, and later in a strong proliferation and formation of cell-to-cell net growing through the tadpole body. Immunohistochemistry analysis of transplanted tadpoles showed a strong expression of vimentin in RFP-positive cells. No co-localization of Sox9 and Sma signals was observed during the first three weeks indicating their dedifferentiation to migratory-active mesenchymal cells recently described in human testicular biopsies.
Trvalý link: http://hdl.handle.net/11104/0269803