G-quadruplex formation in the Oct4 promoter positively regulates Oct4 expression

0473579 - BFÚ 2018 RIV NL eng J - Článek v odborném periodiku
Renčiuk, Daniel - Ryneš, J. - Kejnovská, Iva - Foldynova-Trantirkova, S. - Andaeng, M. - Trantírek, L. - Vorlíčková, Michaela
G-quadruplex formation in the Oct4 promoter positively regulates Oct4 expression.
Biochimica et Biophysica Acta-Gene Regulatory Mechanisms. Roč. 1860, č. 2 (2017), s. 175-183. ISSN 1874-9399. E-ISSN 1876-4320
Grant CEP: GA ČR(CZ) GP14-33947P; GA ČR GAP205/12/0466; GA ČR(CZ) GA15-06785S
Institucionální podpora: RVO:68081707
Klíčová slova: linked polymorphic region * guanine quadruplexes * transcription factor
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.179, rok: 2017

The Oct4 gene codes for a transcription factor that plays a critical role in the maintenance of pluripotency in embryonic and cancer stem cells. Its expression thus has to be tightly regulated. We performed biophysical characterization of the promoter region using a combination of UV absorption, CD, and NMR spectroscopies, native PAGE and chemical probing, which was followed by functional studies involving luciferase reporter assays performed in osteosarcoma and human embryonic stem cell lines.
Trvalý link: http://hdl.handle.net/11104/0270707