Počet záznamů: 1
Isolation and Screening of Lipase Producing Microorganisms from Natural Sources
- 1.0473780 - ÚVGZ 2018 RIV IN eng J - Článek v odborném periodiku
Singh, M. G. - Chandraveer, C. - Tripathi, Abishek
Isolation and Screening of Lipase Producing Microorganisms from Natural Sources.
Indian Journal of Ecology. Roč. 44, č. 1 (2017), s. 19-23. ISSN 0304-5250
Institucionální podpora: RVO:67179843
Klíčová slova: lipase assay * natural sources * screening * submerged fermentation
Obor OECD: Environmental sciences (social aspects to be 5.7)
Lipases are the enzymes that bring about a wide range of bioconversion reactions. Lipases occur widely in nature, but microbial lipases are commercially most significant. Lipase catalyzedtrans-esterification, hydrolysis and esterification are the important class of reactions for technological applications in fats and oil industry, dairy industry, pharmaceuticals and bakery industry. The main reason for the steadily growing interest in lipases is also because of their enantio-selective, regioselective and chemo-selective nature. Enzyme-mediated reactions are attractive alternatives to tedious and expensive chemical methods. Present investigation aimed at bio-prospection of lipase producing microbial isolates from various natural sources. The better enzyme producing microbial isolate for its ability to produce the enzyme was investigated under diverged nutrient conditions of lipase assay. A total of twenty two microbial isolates were isolated from different sources, out of which nine fungal isolates and thirteen bacterial isolates were found to show appreciable lipase producing ability. These cultures were further used to find out their quantitative production of lipase in liquid media with time. It was observed that in majority of the cases, the lipase production increased with time, reaching the maximum on fifth day and decreasing thereafter.
Trvalý link: http://hdl.handle.net/11104/0270915
Počet záznamů: 1