Počet záznamů: 1  

Binding of N-methylscopolamine to the extracellular domain of muscarinic acetylcholine receptors

  1. 1.
    0474008 - FGÚ 2018 RIV GB eng J - Článek v odborném periodiku
    Jakubík, Jan - Randáková, Alena - Zimčík, Pavel - El-Fakahany, E. E. - Doležal, Vladimír
    Binding of N-methylscopolamine to the extracellular domain of muscarinic acetylcholine receptors.
    Scientific Reports. Roč. 7, Jan 16 (2017), č. článku 40381. ISSN 2045-2322. E-ISSN 2045-2322
    Grant CEP: GA ČR(CZ) GBP304/12/G069
    Institucionální podpora: RVO:67985823
    Klíčová slova: muscarinic acetylcholine receptors * N-methylscopolamine * ligand binding * molecular dynamics
    Obor OECD: Physiology (including cytology)
    Impakt faktor: 4.122, rok: 2017

    Interaction of orthosteric ligands with extracellular domain was described at several aminergic G protein-coupled receptors, including muscarinic acetylcholine receptors. The orthosteric antagonists quinuclidinyl benzilate (QNB) and N-methylscopolamine (NMS) bind to the binding pocket of the muscarinic acetylcholine receptor formed by transmembrane a-helices. We show that high concentrations of either QNB or NMS slow down dissociation of their radiolabeled species from all five subtypes of muscarinic acetylcholine receptors, suggesting allosteric binding. The affinity of NMS at the allosteric site is in the micromolar range for all receptor subtypes. Using molecular modelling of the M2 receptor we found that E172 and E175 in the second extracellular loop and N419 in the third extracellular loop are involved in allosteric binding of NMS. Mutation of these amino acids to alanine decreased affinity of NMS for the allosteric binding site confirming results of molecular modelling. The allosteric binding site of NMS overlaps with the binding site of some allosteric, ectopic and bitopic ligands. Understanding of interactions of NMS at the allosteric binding site is essential for correct analysis of binding and action of these ligands.
    Trvalý link: http://hdl.handle.net/11104/0271108

     
     
Počet záznamů: 1  

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