PRC2 Represses Hormone-Induced Somatic Embryogenesis in Vegetative Tissue of Arabidopsis thaliana

0474503 - MBÚ 2018 RIV US eng J - Článek v odborném periodiku
Mozgová, Iva - Munoz-Viana, R. - Hennig, L.
PRC2 Represses Hormone-Induced Somatic Embryogenesis in Vegetative Tissue of Arabidopsis thaliana.
PLoS Genetics. Roč. 13, č. 1 (2017), s. 1-27, č. článku e1006562. ISSN 1553-7404. E-ISSN 1553-7404
Grant CEP: GA ČR(CZ) GJ16-08423Y; GA MŠMT(CZ) LO1416
Institucionální podpora: RVO:61388971
Klíčová slova: PRC2 * SE formation * Arabidopsis thaliana
Obor OECD: Microbiology
Impakt faktor: 5.540, rok: 2017

Many plant cells can be reprogrammed into a pluripotent state that allows ectopic organ development. Inducing totipotent states to stimulate somatic embryo (SE) development is, however, challenging due to insufficient understanding of molecular barriers that prevent somatic cell dedifferentiation. Here we show that Polycomb repressive complex 2 (PRC2)-activity imposes a barrier to hormone-mediated transcriptional reprogramming towards somatic embryogenesis in vegetative tissue of Arabidopsis thaliana. We identify factors that enable SE development in PRC2-depleted shoot and root tissue and demonstrate that the establishment of embryogenic potential is marked by ectopic co-activation of crucial developmental regulators that specify shoot, root and embryo identity. Using inducible activation of PRC2 in PRC2-depleted cells, we demonstrate that transient reduction of PRC2 activity is sufficient for SE formation. We suggest that modulation of PRC2 activity in plant vegetative tissue combined with targeted activation of developmental pathways will open possibilities for novel approaches to cell reprogramming.
Trvalý link: http://hdl.handle.net/11104/0272294