Different roles of CD4, CD8 and gamma delta T-lymphocytes in naive and vaccinated chickens during Salmonella Enteritidis infection

0485640 - BFÚ 2018 RIV DE eng J - Článek v odborném periodiku
Sekelova, Z. - Polanský, O. - Štěpánová, H. - Fedr, Radek - Faldynová, M. - Rychlík, I. - Vlasatiková, L.
Different roles of CD4, CD8 and gamma delta T-lymphocytes in naive and vaccinated chickens during Salmonella Enteritidis infection.
Proteomics. Roč. 17, č. 13-14 (2017), č. článku 1700073. ISSN 1615-9853. E-ISSN 1615-9861
Institucionální podpora: RVO:68081707
Klíčová slova: dendritic cells * activation * cd28
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.532, rok: 2017

Lymphocytes represent the key antigen-specific leukocyte subpopulation. Despite their importance in mounting an immune response, an unbiased description of proteins expressed by chicken lymphocytes has not been presented. In this study, we therefore intravenously infected chickens with Salmonella Enteritidis, sorted CD4, CD8 and gamma delta T-lymphocytes from the spleen by flow cytometry and determined the proteome of each population by LC-MS/MS. CD4 T-lymphocyte characteristic proteins included ubiquitin SUMO-like domain and BAR domain containing proteins. CD8 T-lymphocyte specific proteins were characterized by purine ribonucleoside triphosphate binding and were involved in cell differentiation, cell activation and regulation of programmed cell death. gamma delta T-lymphocyte specific proteins exhibited enrichment of small GTPase of Rab type and GTP binding. Following infection, inducible proteins in CD4 lymphocytes included ribosomal proteins and downregulated proteins localized to the lysosome. CD8 T-lymphocytes induced MCM complex proteins, proteins required for DNA replication and machinery for protein processing in the endoplasmic reticulum. Proteins inducible in gamma delta T-lymphocytes belonged to immune system response, oxidative phosphorylation and the spliceosome. In this study, we predicted the likely events in lymphocyte response to systemic bacterial infection and identified proteins which can be used as markers specific for each lymphocyte subpopulation.
Trvalý link: http://hdl.handle.net/11104/0280606