Development of a high throughput single-particle screening for inorganic semiconductor nanorods as neural voltage sensor

0485882 - ÚOCHB 2018 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Kuo, Y. - Park, K. - Li, J. - Ingargiola, A. - Park, J. - Shvadchak, Volodymyr - Weiss, S.
Development of a high throughput single-particle screening for inorganic semiconductor nanorods as neural voltage sensor.
Proceedings of SPIE. Biosensing and Nanomedicine X. Vol. 10352. Bellingham: SPIE, 2017 - (Mohseni, H.; Agahi, M.; Razeghi, M.), č. článku 103520L. ISBN 978-1-5106-1161-0. ISSN 0277-786X.
[SPIE Nanoscience + Engineering. Biosensing and Nanomedicine /10./. San Diego (US), 06.08.2017-07.08.2017]
Institucionální podpora: RVO:61388963
Klíčová slova: nanoparticles * membrane potential * sensors
Obor OECD: Biophysics

Monitoring membrane potential in neurons requires sensors with minimal invasiveness, high spatial and temporal (sub-ms) resolution, and large sensitivity for enabling detection of sub-threshold activities. While organic dyes and fluorescent proteins have been developed to possess voltage-sensing properties, photobleaching, cytotoxicity, low sensitivity, and low spatial resolution have obstructed further studies. Semiconductor nanoparticles (NPs), as prospective voltage sensors, have shown excellent sensitivity based on Quantum confined Stark effect (QCSE) at room temperature and at single particle level. Both theory and experiment have shown their voltage sensitivity can be increased significantly via material, bandgap, and structural engineering. Based on theoretical calculations, we synthesized one of the optimal candidates for voltage sensors: 12 nm type-II ZnSe/CdS nanorods (NRs), with an asymmetrically located seed. The voltage sensitivity and spectral shift were characterized in vitro using spectrally-resolved microscopy using electrodes grown by thin film deposition, which ”sandwich” the NRs. We characterized multiple batches of such NRs and iteratively modified the synthesis to achieve higher voltage sensitivity (Delta F/F>10%), larger spectral shift (>5 nm), better homogeneity, and better colloidal stability. Using a high throughput screening method, we were able to compare the voltage sensitivity of our NRs with commercial spherical quantum dots (QDs) with single particle statistics. Our method of high throughput screening with spectrally-resolved microscope also provides a versatile tool for studying single particles spectroscopy under field modulation.
Trvalý link: http://hdl.handle.net/11104/0280808