Wheat Rust Diseases

0485924 - ÚEB 2018 RIV US eng M - Část monografie knihy
Steuernagel, B. - Vrána, Jan - Karafiátová, Miroslava - Wulff, B. B. H. - Doležel, Jaroslav
Rapid gene isolation using MutChromSeq.
Wheat Rust Diseases. New York: Humana Press, 2017 - (Periyannan, S.), Roč. 1659 (2017), s. 231-243. ISBN 978-1-4939-7248-7
Grant CEP: GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: Chromosome isolation * fishis * Flow cytometry and chromosome sorting * Gene cloning * Mutational genomics
Obor OECD: Genetics and heredity (medical genetics to be 3)
http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=28856655

MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.
Trvalý link: http://hdl.handle.net/11104/0280841