Improved demethylation in ecological epigenetic experiments: Testing a simple and harmless foliar demethylation application

0490426 - BÚ 2019 RIV GB eng J - Článek v odborném periodiku
Puy, J. - Dvořáková, H. - Carmona, C. P. - de Bello, Francesco - Hiiesalu, I. - Latzel, Vít
Improved demethylation in ecological epigenetic experiments: Testing a simple and harmless foliar demethylation application.
Methods in Ecology and Evolution. Roč. 9, č. 3 (2018), s. 744-753. ISSN 2041-210X. E-ISSN 2041-2096
Grant CEP: GA ČR(CZ) GA17-11281S; GA ČR(CZ) GA16-15012S; GA ČR(CZ) GA14-06802S
Institucionální podpora: RVO:67985939
Klíčová slova: 5-azacytidine * competition * demethylation
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 7.099, rok: 2018

We compared a novel method of demethylating plants, based on periodical spraying of 5-azaC aqueous solution on established seedlings, with the previous method in which seeds were germinated directly in 5-azaC solution.We found that the spray application had similar DNA-demethylating efficiency than the germination method, particularly in the earlier phases of plant development, but without unwanted effects. The spray application method did not reduce plant growth and performance compared to untreated plants, as opposed to the traditional method which showed reduced growth.
Trvalý link: http://hdl.handle.net/11104/0284653