TATAA courses – 1 day Sample preparation and quality control of nucleic acids\n\n

0496246 - BTÚ 2019 RIV eng U - Uspořádání akce
Kubista, Mikael - Valihrach, Lukáš - Smolík, Ondřej - Androvič, Peter - Rohlová, Eva - Švec, David
TATAA courses – 1 day Sample preparation and quality control of nucleic acids

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[Vestec, BIOCEV, 18.04.2018-18.04.2018, (W-WRD 12/9)]
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: qPCR * quality control * reverese transcription
Obor OECD: Biochemistry and molecular biology

One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course avers : Overview of nucleic acid extraction methods. How to properly determine the concentration of purified nucleic acids. Storage of biological samples and purified nucleic acids. Quality control of purified material. Integrity of purified RNA. How to test for the presence of inhibitors.
Trvalý link: http://hdl.handle.net/11104/0289061