Capillary electrophoresis with preparative isoelectric focusing preconcentration for sensitive determination of amphotericin B in human blood serum

0501010 - ÚIACH 2020 RIV NL eng J - Článek v odborném periodiku
Šalplachta, Jiří - Horká, Marie - Šlais, Karel
Capillary electrophoresis with preparative isoelectric focusing preconcentration for sensitive determination of amphotericin B in human blood serum.
Analytica Chimica Acta. Roč. 1053, APR (2019), s. 162-168. ISSN 0003-2670. E-ISSN 1873-4324
Grant CEP: GA ČR(CZ) GA16-03749S; GA MZd(CZ) NV16-29916A; GA MV VI20172020069
Institucionální podpora: RVO:68081715
Klíčová slova: capillary zone electrophoresis * Amphotericin B * blood * sample preparation
Obor OECD: Analytical chemistry
Impakt faktor: 5.977, rok: 2019
Způsob publikování: Omezený přístup

Amphotericin B (AmB) is still, despite its severe nephrotoxicity, the first-line agent in the management of serious systemic fungal infections. A sensitive and reliable method is therefore required to control AmB concentration in body fluids of a patient. This study demonstrates the potential of the off-line combination of preparative isoelectric focusing (IEF) with capillary isoelectric focusing (CIEF) or capillary zone electrophoresis (CZE) in the determination of AmB in human blood serum. The required value of the isoelectric point of AmB was determined to be 6.1 using the CIEF technique. Preparative IEF served as a pre-separation and concentration technique. The pH gradient was traced by colored low molecular pI markers. The collected fraction with AmB was easily processed and then analyzed by CIEF and CZE. Tens of picograms of AmB in human blood serum sample can be determined by a combination of preparative IEF with CZE. The method was linear in the AmB concentration range of 0.3e600 ngmL 1. The recovery ranged from 93% to 98%.
Trvalý link: http://hdl.handle.net/11104/0293037