Počet záznamů: 1  

Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry

  1. 1.
    0501578 - BFÚ 2019 RIV DE eng J - Článek v odborném periodiku
    Malina, Jaroslav - Brabec, Viktor
    Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry.
    ChemistrySelect. Roč. 3, č. 46 (2018), s. 13076-13080. ISSN 2365-6549. E-ISSN 2365-6549
    Grant CEP: GA ČR GA17-09436S
    Institucionální podpora: RVO:68081707
    Klíčová slova: polymerase * damage * nucleoside * energetics
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 1.716, rok: 2018

    Previous reports indicated that when an abasic (apurinic/apyrimidinic, AP) site is bypassed by DNA polymerases, dATP is preferentially inserted. Here we evaluate, using differential scanning calorimetry, the thermodynamic changes associated with incorporation of N-6-methyl-dATP opposite an AP site, dCMP, and dTMP during simulated DNA polymerization. The results confirm that AP sites block DNA polymerases one nucleotide prior to the lesion. Thermodynamic data imply that the propensity of N-6-methyl-dAMP for elongation, when incorporated opposite an AP site, is higher than that of dAMP in agreement with a higher promutagenic potential of N-6-methyl-dATP if placed opposite a non-instructional DNA lesion, such as an AP site.
    Trvalý link: http://hdl.handle.net/11104/0293580

     
     
Počet záznamů: 1  

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