Irradiation by gamma-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and gamma H2AX

0501694 - BFÚ 2019 RIV FR eng J - Článek v odborném periodiku
Bártová, Eva - Lochmanová, G. - Legartová, Soňa - Suchánková, Jana - Fedr, Radek - Krejčí, Jana - Zdráhal, Z.
Irradiation by gamma-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and gamma H2AX.
Biochimie. Roč. 154, NOV 2018 (2018), s. 86-98. ISSN 0300-9084. E-ISSN 1638-6183
Grant CEP: GA ČR(CZ) GA18-07384S
Grant ostatní: AV ČR(CZ) StrategieAV21/7
Program: StrategieAV
Institucionální podpora: RVO:68081707
Klíčová slova: dna-damage response * double-strand breaks * histone h3 * saccharomyces-cerevisiae
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.362, rok: 2018

Histone posttranslational modifications regulate diverse nuclear functions, including DNA repair. Here, we use mass spectrometry, western blotting, immunohistochemistry and advanced confocal microscopy in order to show radiation-specific changes in the histone signature. We studied wild-type mouse embryonic stem cells (mESCs) and mESCs with a depletion of histone deacetylase 1 (HDAC1), which plays a role in DNA repair. Irradiation by gamma-rays increased the S139 phosphorylation of histone H2AX but reduced the level of the H3K9-R17 peptide, which contains S10 phosphorylation (H3S10ph). On an individual cellular level, H3S10ph was low in highly gamma H2AX-positive UV laser-induced DNA lesions, and this nuclear distribution pattern was not changed by HDAC1 depletion. Despite this fact, spontaneous gamma H2AX-positive DNA lesions colocalized with large H3S10ph-positive nuclear bodies that appear in the G2 phase of the cell cycle. Similarly, by FLIM-FRET analysis, we observed an interaction between H3S10ph and gamma H2AX in the G2 phase. However, this interaction was reduced when cells were exposed to gamma-rays. A mutual link between H3S10ph and gamma H2AX was not observed in the G1 phase of the cell cycle. Together, our data show that despite the fact that H3S10ph is not directly involved in DNA repair, a decrease in H3S10 phosphorylation and weakened interaction between H3S10ph and gamma H2AX is a result of radiation-induced damage of the genome. In this case, gamma-irradiation also decreased the number of cells in the G1 phase, characterized by no interaction between H3S10ph and gamma H2AX. (C) 2018 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0293705