The role of alpha beta T-cells in spontaneous regression of melanoma tumors in swine

0503686 - ÚŽFG 2020 RIV GB eng J - Článek v odborném periodiku
Čížková, Jana - Šinkorová, Z. - Strnadová, K. - Červinková, Monika - Horák, Vratislav - Šinkora, J. - Štěpánová, Kateřina - Šinkora, Marek
The role of alpha beta T-cells in spontaneous regression of melanoma tumors in swine.
Developmental and Comparative Immunology. Roč. 92, MAR (2019), s. 60-68. ISSN 0145-305X. E-ISSN 1879-0089
Grant CEP: GA MŠMT(CZ) LO1609; GA ČR(CZ) GA16-09296S
Institucionální podpora: RVO:67985904 ; RVO:61388971
Klíčová slova: porcine immune system * lymphocyte subpopulations * cell surface molecules
Obor OECD: Zoology; Zoology (MBU-M)
Impakt faktor: 3.192, rok: 2019
Způsob publikování: Omezený přístup
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=67855917795

Using a porcine model, we describe Melanoma-Associated CD4(+)CD8(hi) T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4(+)CD8(hi) phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4(+)CD8(hi) TIL have a similar expression of selected markers indicating that they rep(re)sent effector/memory alpha beta T-cell subset. Moreover, although TIL also contain CD4(-)CD8(hi) T-cells, only CD4(+)CD8(hi) TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.
Trvalý link: http://hdl.handle.net/11104/0295495