Effects of post-mortem and physical degradation on RNA integrity and quality

0523050 - BTÚ 2020 RIV NL eng J - Článek v odborném periodiku
Šídová, Monika - Tománková, Silvie - Abaffy, Pavel - Kubista, Mikael - Šindelka, Radek
Effects of post-mortem and physical degradation on RNA integrity and quality.
Biomolecular Detection and Quantification. Roč. 5, September 2015 (2015), s. 3-9. ISSN 2214-7535
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: Degradation * RNA integrity * RNA quality * rqi
Obor OECD: Biochemistry and molecular biology
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S2214753515300048?via%3Dihub

The precision and reliability of quantitative nucleic acid analysis depends on the quality of the sample analyzed and the integrity of the nucleic acids. The integrity of RNA is currently primarily assessed by the analysis of ribosomal RNA, which is the by far dominant species. The extrapolation of these results to mRNAs and microRNAs, which are structurally quite different, is questionable. Here we show that ribosomal and some nucleolar and mitochondrial RNAs, are highly resistant to naturally occurring post-mortem degradation, while mRNAs, although showing substantial internal variability, are generally much more prone to nucleolytic degradation. In contrast, all types of RNA show the same sensitivity to heat. Using qPCR assays targeting different regions of mRNA molecules, we find no support for 5' or 3' preferentiality upon post-mortem degradation.
Trvalý link: http://hdl.handle.net/11104/0307459