Advances in Disease Models

0523240 - ÚMG 2020 RIV CZ eng M - Část monografie knihy
Pečenka, Vladimír - Pajer, Petr - Karafiát, Vít - Dvořák, Michal
Exploring avian cancer genome with insertional mutagenesis screens using myeloblastosis associated viruses.
Advances in Disease Models. Praha: OPTIO CZ, 2019 - (Bartůněk, P.), s. 189-206. ISBN 978-80-88011-06-4
Grant CEP: GA MŠMT LO1419
Institucionální podpora: RVO:68378050
Klíčová slova: chicken retrovirus myeloblastosis-associated virus-2 * insertional mutagenesis * oncogenesis
Obor OECD: Virology

We have established a new model for studying cancer genetics based on insertional mutagenesis by chicken retrovirus myeloblastosis-associated virus-2 (MAV-2) and tumor-promoting agents that target tumorigenesis into individual organs. Using high-performance iPCR we analyzed a large number of chicken tumors, pinpointed common provirus-insertion sites and associated them with candidate cancer genes. So far, we have defined the principal oncogenes driving formation of chicken nephroblastomas (FOXP1, PLAG1, TWIST or HRAS), lung hemangiosarcomas (FRK), liver hemangiosarcomas (HRAS), hepatocarcinomas (EGFR), and cholangiocarcinomas (HGFR or MST1R)(1-4). All of them are now proven human oncogenes, in three cases (TWIST, FOXP1, FRK), our findings preceded the discovery of their involvement in human tumors. This shows that non-mouse species are good alternative models for studying oncogenesis with outcomes applicable to human medicine.

Trvalý link: http://hdl.handle.net/11104/0307612