The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms

0524258 - BFÚ 2021 RIV CH eng J - Článek v odborném periodiku
Porubiakova, Otilia - Bohalova, Natalia - Inga, A. - Vadovičová, Natália - Coufal, Jan - Fojta, Miroslav - Brázda, Václav
The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms.
International Journal of Molecular Sciences. Roč. 21, č. 1 (2020), č. článku 127. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GA18-15548S; GA MŠMT EF15_003/0000477
Institucionální podpora: RVO:68081707
Klíčová slova: dna-binding domain * tumor-suppressor * inverted repeats * promoter
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.924, rok: 2020
Způsob publikování: Open access
file:///C:/Users/user/Downloads/ijms-21-00127.pdf

p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53 alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53 alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53 alpha isoforms.
Trvalý link: http://hdl.handle.net/11104/0308640