Počet záznamů: 1  

Structural characterization of the interaction between the C-terminal domain of the influenza polymerase PA subunit and an optimized small peptide inhibitor

  1. 1.
    0536615 - ÚOCHB 2022 RIV NL eng J - Článek v odborném periodiku
    Hejdánek, Jakub - Radilová, Kateřina - Pachl, Petr - Hodek, Jan - Machara, Aleš - Weber, Jan - Řezáčová, Pavlína - Konvalinka, Jan - Kožíšek, Milan
    Structural characterization of the interaction between the C-terminal domain of the influenza polymerase PA subunit and an optimized small peptide inhibitor.
    Antiviral Research. Roč. 185, Jan (2021), č. článku 104971. ISSN 0166-3542. E-ISSN 1872-9096
    Grant CEP: GA MŠMT(CZ) EF16_019/0000729; GA MŠMT(CZ) LM2018133
    Institucionální podpora: RVO:61388963
    Klíčová slova: antiviral peptides * influenza a polymerase * protein-protein interaction * AlphaScreen
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 10.103, rok: 2021
    Způsob publikování: Open access
    https://doi.org/10.1016/j.antiviral.2020.104971

    Influenza viruses can cause severe respiratory infections in humans, leading to nearly half a million deaths worldwide each year. Improved antiviral drugs are needed to address the threat of development of novel pandemic strains. Current therapeutic interventions target three key proteins in the viral life cycle: neuraminidase, the M2 channel and RNA-dependent-RNA polymerase. Protein-protein interactions between influenza polymerase subunits are potential new targets for drug development. Using a newly developed assay based on AlphaScreen technology, we screened a peptide panel for protein-protein interaction inhibitors to identify a minimal PB1 subunit-derived peptide that retains high inhibition potential and can be further modified. Here, we present an X-ray structure of the resulting decapeptide bound to the C-terminal domain of PA polymerase subunit from pandemic isolate A/California/07/2009 H1N1 at 1.6 Å resolution and discuss its implications for the design of specific, potent influenza polymerase inhibitors.
    Trvalý link: http://hdl.handle.net/11104/0316256

     
     
Počet záznamů: 1  

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