Differential Scaling of Gene Expression with Cell Size May Explain Size Control in Budding Yeast

0539318 - ÚEM 2021 RIV US eng J - Článek v odborném periodiku
Chen, Y.P. - Zhao, G. - Zahumenský, Jakub - Honey, S. - Futcher, B.
Differential Scaling of Gene Expression with Cell Size May Explain Size Control in Budding Yeast.
Molecular Cell. Roč. 78, č. 2 (2020), s. 359-370. ISSN 1097-2765. E-ISSN 1097-4164
Institucionální podpora: RVO:68378041
Klíčová slova: saccharomyces-cerevisiae * transcription-factor * G1 cyclins
Obor OECD: Cell biology
Impakt faktor: 17.970, rok: 2020
Způsob publikování: Omezený přístup
https://www.cell.com/molecular-cell/fulltext/S1097-2765(20)30160-X?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS109727652030160X%3Fshowall%3Dtrue

Yeast cells must grow to a critical size before committing to division. It is unknown how size is measured. We find that as cells grow, mRNAs for some cell-cycle activators scale faster than size, increasing in concentration, while mRNAs for some inhibitors scale slower than size, decreasing in concentration. Size-scaled gene expression could cause an increasing ratio of activators to inhibitors with size, triggering cell-cycle entry. Consistent with this, expression of the CLN2 activator from the promoter of the WHI5 inhibitor, or vice versa, interfered with cell size homeostasis, yielding a broader distribution of cell sizes. We suggest that size homeostasis comes from differential scaling of gene expression with size. Differential regulation of gene expression as a function of cell size could affect many cellular processes.
Trvalý link: http://hdl.handle.net/11104/0317002