Počet záznamů: 1
Zebrafish Kit ligands cooperate with erythropoietin to promote erythroid cell expansion
- 1.0539675 - ÚMG 2021 RIV US eng J - Článek v odborném periodiku
Oltová, Jana - Svoboda, Ondřej - Machoňová, Olga - Svatoňová, Petra - Traver, D. - Kolář, Michal - Bartůněk, Petr
Zebrafish Kit ligands cooperate with erythropoietin to promote erythroid cell expansion.
Blood Advances. Roč. 4, č. 23 (2020), s. 5915-5924. ISSN 2473-9529. E-ISSN 2473-9537
Grant CEP: GA ČR GA16-21024S; GA MZd NV19-07-00412
Institucionální podpora: RVO:68378050
Klíčová slova: c-kit * progenitor cells * terminal differentiation * glucocorticoid-receptor * clonal analysis * growth-factor * gene-product * hematopoiesis * expression * chicken
Obor OECD: Cell biology
Impakt faktor: 6.799, rok: 2020
Způsob publikování: Open access
https://ashpublications.org/bloodadvances/article/4/23/5915/474371/Zebrafish-Kit-ligands-cooperate-with
Kit ligand (Kitlg) is pleiotropic cytokine with a prominent role in vertebrate erythropoiesis. Although the role of Kitlg in this process has not been reported in Danio rerio (zebrafish), in the present study we show that its function is evolutionarily conserved. Zebrafish possess 2 copies of Kitlg genes (Kitlga and Kitlgb) as a result of whole-genome duplication. To determine the role of each ligand in zebrafish, we performed a series of ex vivo and in vivo gain- and loss-of-function experiments. First, we tested the biological activity of recombinant Kitlg proteins in suspension culture from zebrafish whole-kidney marrow, and we demonstrate that Kitlga is necessary for expansion of erythroid progenitors ex vivo. To further address the role of kitlga and kitlgb in hematopoietic development in vivo, we performed gain-of-function experiments in zebrafish embryos, showing that both ligands cooperate with erythropoietin (Epo) to promote erythroid cell expansion. Finally, using the kita mutant (kita(b5/b5) or sparse), we show that the Kita receptor is crucial for Kitlga/b cooperation with Epo in erythroid cells. In summary, using optimized suspension culture conditions with recombinant cytokines (Epo, Kitlga), we report, for the first time, ex vivo suspension cultures of zebrafish hematopoietic progenitor cells that can serve as an indispensable tool to study normal and aberrant hematopoiesis in zebrafish. Furthermore, we conclude that, although partial functional diversification of Kit ligands has been described in other processes, in erythroid development, both paralogs play a similar role, and their function is evolutionarily conserved.
Trvalý link: http://hdl.handle.net/11104/0317388
Počet záznamů: 1