Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies

0542811 - ÚMCH 2022 RIV US eng J - Článek v odborném periodiku
Williams, W. B. - Meyerhoff, R. R. - Edwards, R. J. - Li, H. - Manne, K. - Nicely, N. I. - Henderson, R. - Zhou, Y. - Janowska, K. - Mansouri, K. - Gobeil, S. - Evangelous, T. - Hora, B. - Berry, M. - Abuahmad, A. Y. - Sprenz, J. - Deyton, M. - Stalls, V. - Kopp, M. - Hsu, A. L. - Borgnia, M. J. - Stewart-Jones, G. B. E. - Lee, M. S. - Bronkema, N. - Moody, M. A. - Wiehe, K. - Bradley, T. - Alam, S. M. - Parks, R. J. - Foulger, A. - Oguin, T. - Sempowski, G. D. - Bonsignori, M. - LaBranche, C. C. - Montefiori, D. C. - Seaman, M. - Santra, S. - Perfect, J. - Francica, J. R. - Lynn, G. M. - Aussedat, B. - Walkowicz, W. E. - Laga, Richard - Kelsoe, G. - Saunders, K. O. - Fera, D. - Kwong, P. D. - Seder, R. A. - Bartesaghi, A. - Shaw, G. M. - Acharya, P. - Haynes, B. F.
Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.
Cell. Roč. 184, č. 11 (2021), s. 2955-2972.e25. ISSN 0092-8674. E-ISSN 1097-4172
Institucionální podpora: RVO:61389013
Klíčová slova: natural Abs * glycan-dependent Ab binding * HIV-1 Env glycans
Obor OECD: Polymer science
Impakt faktor: 66.850, rok: 2021
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S0092867421005778?via%3Dihub

Natural antibodies (Abs) can target host glycans on the surface of pathogens. We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen. 2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization. Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques. FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike. FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans. Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.
Trvalý link: http://hdl.handle.net/11104/0320309