Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State

0544239 - ÚFCH JH 2022 RIV NL eng J - Článek v odborném periodiku
Riegerová, Petra - Brejcha, Jindřich - Bezděková, D. - Chum, Tomáš - Mašínová, Eva - Čermáková, Nikola - Ovsepian, S. V. - Cebecauer, Marek - Štefl, Martin
Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State.
Journal of Alzheimer's Disease. Roč. 82, č. 2 (2021), s. 485-491. ISSN 1387-2877. E-ISSN 1875-8908
Grant CEP: GA ČR(CZ) GJ19-08304Y
Grant ostatní: Akademie věd - GA AV ČR(CZ) L200401901
Institucionální podpora: RVO:61388955
Klíčová slova: AβPP * Alzheimer’s disease * differentiation
Obor OECD: Cell biology
Impakt faktor: 4.160, rok: 2021
Způsob publikování: Open access

Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.

Trvalý link: http://hdl.handle.net/11104/0321269