Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro

0550825 - MBÚ 2022 RIV CZ eng J - Článek v odborném periodiku
Kolesárová, A. - Michalcová, K. - Roychoudhury, S. - Baldovská, S. - Tvrdá, E. - Vašíček, J. - Chrenek, P. - Sanislo, L. - Křen, Vladimír
Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro.
Physiological Research. Roč. 70, č. 5 (2021), s. 745-754. ISSN 0862-8408. E-ISSN 1802-9973
Institucionální podpora: RVO:61388971
Klíčová slova: Flavonoid * Granulosa cells * Antioxidant * Steroid hormones * Transforming growth factor
Obor OECD: Pharmacology and pharmacy
Impakt faktor: 2.139, rok: 2021
Způsob publikování: Open access
https://www.biomed.cas.cz/physiolres/pdf/2021/70_745.pdf

This study aimed to examine the effect of dietary flavonoid isoquercitrin on ovarian granulosa cells using the immortalized human cell line HGL5. Cell viability, survival, apoptosis, release of steroid hormones 17 beta-estradiol and progesterone, and human transforming growth factor-beta 2 (TGF-beta 2) and TGF-beta 2 receptor as well as intracellular reactive oxygen species (ROS) generation were investigated after isoquercitrin treatment at the concentration range of 5-100 mu g.ml(-1). It did not cause any significant change (p>0.05) in cell viability as studied by AlamarBlue assay in comparison to control. No significant change was observed (p>0.05) in the proportion of live, dead and apoptotic cells as revealed by apoptotic assay using flow cytometry. Similarly, the release of 17 beta-estradiol, progesterone, TGF-beta 2 and its receptor were not affected significantly (p>0.05) by isoquercitrin as detected by ELISA, in comparison to control. Except for the highest concentration of 100 mu g.ml(-1), which led to oxidative stress, isoquercitrin exhibited antioxidative activity at lower concentration used in the study (5, 10, 25, and 50 mu g.ml(-1)) by hampering the production of intracellular ROS, in comparison to control, as detected by chemiluminescence assay (p<0.05). Findings of the present study indicate an existence of the antioxidative pathway that involves inhibition of intracellular ROS generation by isoquercitrin in human ovarian granulosa cells.
Trvalý link: http://hdl.handle.net/11104/0326133