Freeze-fracturing of microbes producing biopolymers at liquid Helium temperature: cryo-SEM application in biotechnology

0552527 - ÚPT 2022 RIV US eng A - Abstrakt
Hrubanová, Kamila - Mrázová, Kateřina - Urban, Pavel - Krutil, Vojtěch - Skoupý, Radim - Obruča, S. - Krzyžánek, Vladislav
Freeze-fracturing of microbes producing biopolymers at liquid Helium temperature: cryo-SEM application in biotechnology.
Microscopy and Microanalysis. Cambridge University Press. Roč. 27, S1 (2021), s. 3164-3166. ISSN 1431-9276. E-ISSN 1435-8115
Grant CEP: GA ČR(CZ) GA19-20697S
Grant ostatní: AV ČR(CZ) MSM100652102
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institucionální podpora: RVO:68081731
Klíčová slova: cryo-SEM * biopolymers * liquid Helium * biotechnology
Obor OECD: Electrical and electronic engineering
https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/freezefracturing-of-microbes-producing-biopolymers-at-liquid-helium-temperature-cryosem-application-in-biotechnology/50BC0DD9DE37425C157D7C740B5DE39A

Interesting morphologies can be observed when microorganisms containing biopolymeric particles are fractured at very low temperatures far below the glass transition temperatures of this polymer. Some biopolymers such as Polyhydroxyalkanoates (PHAs) can be stretched extensively when fractured at -120°C. This property is different when the same polymer is chemically prepared. In our study was our challenge is to break the sample at an even lower temperature and observe the fracture plane by cryogenic electron microscopy. The new cooling system was developed and applied in several experiments described in this study.
Trvalý link: http://hdl.handle.net/11104/0327637