Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology

0555493 - ÚMG 2022 RIV GB eng J - Článek v odborném periodiku
Soukal, P. - Hrdá, Š. - Karnkowska, A. - Milanowski, R. - Szabová, J. - Hradilová, Miluše - Strnad, Hynek - Vlček, Čestmír - Čepička, I. - Hampl, V.
Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology.
Scientific Reports. Roč. 11, č. 1 (2021), č. článku 13070. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA MŠMT(CZ) LQ1604
Institucionální podpora: RVO:68378050
Klíčová slova: phylogenetic analysis * energy-metabolism * sequence * introns * genome * rna * alignment * proteins * gracilis * identification
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impakt faktor: 4.997, rok: 2021
Způsob publikování: Open access
https://www.nature.com/articles/s41598-021-92174-3

Euglenids represent a group of protists with diverse modes of feeding. To date, only a partial genomic sequence of Euglena gracilis and transcriptomes of several phototrophic and secondarily osmotrophic species are available, while primarily heterotrophic euglenids are seriously undersampled. In this work, we begin to fill this gap by presenting genomic and transcriptomic drafts of a primary osmotroph, Rhabdomonas costata. The current genomic assembly length of 100 Mbp is 14x smaller than that of E. gracilis. Despite being too fragmented for comprehensive gene prediction it provided fragments of the mitochondrial genome and comparison of the transcriptomic and genomic data revealed features of its introns, including several candidates for nonconventional types. A set of 39,456 putative R. costata proteins was predicted from the transcriptome. Annotation of the mitochondrial core metabolism provides the first data on the facultatively anaerobic mitochondrion of R. costata, which in most respects resembles the mitochondrion of E. gracilis with a certain level of streamlining. R. costata can synthetise thiamine by enzymes of heterogenous provenances and haem by a mitochondrial-cytoplasmic C4 pathway with enzymes orthologous to those found in E. gracilis. The low percentage of green algae-affiliated genes supports the ancestrally osmotrophic status of this species.
Trvalý link: http://hdl.handle.net/11104/0330000