Rapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma

0553498 - MBÚ 2023 RIV US eng J - Článek v odborném periodiku
Zemanová, N. - Anzenbacher, P. - Hudcovic, Tomáš - Anzenbacherová, E.
Rapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma.
Journal of Chromatographic Science. Roč. 60, č. 1 (2022), s. 81-87. ISSN 0021-9665. E-ISSN 1945-239X
Grant CEP: GA ČR(CZ) GA19-08294S
Institucionální podpora: RVO:61388971
Klíčová slova: performance liquid-chromatography * saliva * assay * validation
Obor OECD: Immunology
Impakt faktor: 1.3, rok: 2022
Způsob publikování: Open access
https://academic.oup.com/chromsci/article-abstract/60/1/81/6238591?redirectedFrom=fulltext&login=true

Metronidazole is a drug used to treat bacterial and protozoan infections. Nowadays, it is one of the most frequently prescribed drugs worldwide. The main aim of this paper is to present a rapid, reliable and simple high-performance liquid chromatography (HPLC) method to determine metronidazole along with its primary metabolite, 2-hydroxymetronidazole, in plasma or serum using paracetamol as an internal standard. A total of 100% methanol was used to denature plasma proteins. After centrifugation, the supernatant was evaporated under nitrogen flow. The samples were dissolved in the mobile phase and injected into a Li-Chrospher RP-18 column. A total of 10 mmol/L NaH2PO4: acetonitrile (90:10, v/v) solution with a flow rate of 1 mL/min was used as the mobile phase. Metronidazole and 2-hydroxymetronidazole were detected at two different wavelengths at 320 nm and 311 nm, respectively. The method is characterized by high precision (relative standard deviation % < 6). The method was used for the determination of metronidazole and 2-hydroxymetronidazole in murine blood using small amounts of plasma (<= 100 mu L).
Trvalý link: http://hdl.handle.net/11104/0331877