- Author
-
R. Hickman
M.C. Van Verk
A.J.H. Van Dijken
M. Pereira Mendes
I.A. Vroegop-Vos
L. Caarls
M. Steenbergen
I. Van der Nagel
G.-J. Wesselink
A. Jironkin
A. Talbot
J. Rhodes
M. De Vries
R.C. Schuurink
K. Denby
C.M.J. Pieterse
S.C.M. Van Wees - Date
- 9-2017
- Title
- Architecture and Dynamics of the Jasmonic Acid Gene Regulatory Network
- Journal
- The Plant Cell
- Volume | Issue number
- 29 | 9
- Pages (from-to)
- 2086-2105
- Number of pages
- 20
- Document type
- Article
- Faculty
- Faculty of Science (FNWI)
- Institute
- Swammerdam Institute for Life Sciences (SILS)
- Abstract
-
Jasmonic acid (JA) is a critical hormonal regulator of plant growth and defense. To advance our understanding of the architecture and dynamic regulation of the JA gene regulatory network, we performed a high-resolution RNA-seq time series of methyl JA-treated Arabidopsis thaliana at 15 time points over a 16-h period. Computational analysis showed that methyl JA (MeJA) induces a burst of transcriptional activity, generating diverse expression patterns over time that partition into distinct sectors of the JA response targeting specific biological processes. The presence of transcription factor (TF) DNA binding motifs correlated with specific TF activity during temporal MeJA-induced transcriptional reprogramming. Insight into the underlying dynamic transcriptional regulation mechanisms was captured in a chronological model of the JA gene regulatory network. Several TFs, including MYB59 and bHLH27, were uncovered as early network components with a role in pathogen and insect resistance. Analysis of subnetworks surrounding the TFs ORA47, RAP2.6L, MYB59, and ANAC055, using transcriptome profiling of overexpressors and mutants, provided insights into their regulatory role in defined modules of the JA network. Collectively, our work illuminates the complexity of the JA gene regulatory network, pinpoints and validates previously unknown regulators, and provides a valuable resource for functional studies on JA signaling components in plant defense and development.
- URL
- go to publisher's site
- Language
- English
- Note
- With supplementary materials
- Persistent Identifier
- https://hdl.handle.net/11245.1/fd948715-24c1-4024-bad8-4118ad9ff820
- Downloads
-
2086.full(Final published version)
- Supplementary materials
-
TPC2016-00958-LSBR2_Supplemental_Data
TPC2016-00958-LSBR2_Supplemental_File_1
TPC2016-00958-LSBR2_Supplemental_Dataset_1
TPC2016-00958-LSBR2_Supplemental_Dataset_2
TPC2016-00958-LSBR2_Supplemental_Dataset_3
TPC2016-00958-LSBR2_Supplemental_Dataset_4
TPC2016-00958-LSBR2_Supplemental_Dataset_5
TPC2016-00958-LSBR2_Supplemental_Dataset_6
TPC2016-00958-LSBR2_Supplemental_Dataset_7
TPC2016-00958-LSBR2_Supplemental_Dataset_8
TPC2016-00958-LSBR2_Supplemental_Dataset_9
TPC2016-00958-LSBR2_Supplemental_Dataset_10
TPC2016-00958-LSBR2_Supplemental_Dataset_11
TPC2016-00958-LSBR2_Supplemental_Dataset_12
TPC2016-00958-LSBR2_Supplemental_Dataset_13
TPC2016-00958-LSBR2_Supplemental_Dataset_14
TPC2016-00958-LSBR2_Supplemental_Dataset_15
TPC2016-00958-LSBR2_Supplemental_Dataset_16
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