- Author
-
A.E.D. van Breevoort
- Title
- Tuning the SNAREs that regulate Weibel-Palade body exocytosis
- Supervisors
-
J.J. Voorberg
- Co-supervisors
-
R. Bierings
M. Fernandez-Borja - Award date
- 18 June 2014
- Number of pages
- 159
- ISBN
- 9789461824318
- Document type
- PhD thesis
- Faculty
- Faculty of Medicine (AMC-UvA)
- Abstract
-
Endothelial cells form the inner lining of the vessel wall and provide a barrier between circulating blood and the underlying tissue. The endothelium participates in maintenance of vascular homeostasis and facilitates rapid responses to environmental changes such as inflammation or vascular damage. A cocktail of bioactive components participating in these processes are stored in endothelial-specific storage organelles, designated Weibel-Palade bodies (WPBs). The main component of WPBs is von Willebrand factor (VWF). VWF functions in arrest of bleeding by capturing platelets thereby promoting thrombus formation at sites of vascular injury. Furthermore, VWF acts as a carrier for coagulation factor VIII. Several other mediators of hemostasis, inflammation and angiogenesis are co-stored with VWF in WPBs and are secreted into the vascular lumen upon WPB exocytosis. In this thesis we aim to obtain a better understanding of the composition of WPBs and the molecular mechanisms that regulate the release of WPB components into the bloodstream.
- Note
- Research conducted at: Sanquin-Landsteiner Laboratory AMC
- Persistent Identifier
- https://hdl.handle.net/11245/1.418231
- Downloads
-
Thesis
Cover
Title pages
Contents
Chapter 1: General introduction
Chapter 2: Proteomic screen identifies IGFBP7 as a novel component of endothelial cell-specific Weibel-Palade bodies
Chapter 3: Impaired VWF release in EIEE4 ex vivo endothelial cells identifies STXBP1 as a positive regulator of Weibel-Palade body exocytosis
Chapter 4: Weibel-Palade body-associated SNARE syntaxin-3 controls basal VWF release
Chapter 5: Syntaxin binding protein 5 (STXBP5) inhibits Weibel-Palade body exocytosis through its carboxy-terminal VAMP-like domain
Chapter 6: General discussion
Summary
Samenvatting
Curriculum vitae
List of publications
Dankwoord
Disclaimer/Complaints regulations
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.