A CRISPR/Cas9 system adapted for gene editing in marine algae
Journal article, Peer reviewed
Åpne
Permanent lenke
http://hdl.handle.net/11250/2427015Utgivelsesdato
2016Metadata
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- Institutt for biologi [2500]
- Publikasjoner fra CRIStin - NTNU [36793]
Sammendrag
Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.