Rats were divided into 5 groups to study the effects of bicuculline-induced convulsions and of some anticonvulsive drugs on the initial stage of glycerol incorporation into cerebellar lipids. Groups were: (a) controls; (b) treated with sodium valproate (400 mg./kg); (c) treated with bicuculline (25 micromol./kg); (d) treated with diazepam (2 mg/kg); (e) treated with diphenylhydantoin (75 mg./kg). Only rats of group (c) had seizures which lasted until the sacrifice ofthe animals. Drugs were administered intraperitoneally 25 min before the isotope (20 micro Ci of [2-3H]glycerol, which was injected into brain ventriculi), except bicuculline whose administration was simultaneous to that of labeled glycerol. Rats were sacrificed 12 min after the injection of the radioactive precursor. Cerebella were taken and lipid and water-soluble extracts examined. The treatment very much influenced the fate oflabeled, injected glycerol. Indeed, the radioactivity recovered in water-soluble extracts increased dramatically during convulsions or following the administration of any tested drugs, whereas the radioactivity incorporated into lipid decreased. The distribution of lipid radioactivity between neutral glycerolipid and glycerophospholipid was affected by seizures but not by anticonvulsive drugs. Therefore the performed treatments produced two effects: one was common to all tested drugs, whereas the other could be observed only after bicuculline.
CEREBELLAR LIPID METABOLISM IS AFFECTED BYBICUCULLINE-INDUCED SEIZURES AND BY SOMEANTICONVULSIVE DRUGS
ARIENTI, Giuseppe;CORAZZI, Lanfranco
1989
Abstract
Rats were divided into 5 groups to study the effects of bicuculline-induced convulsions and of some anticonvulsive drugs on the initial stage of glycerol incorporation into cerebellar lipids. Groups were: (a) controls; (b) treated with sodium valproate (400 mg./kg); (c) treated with bicuculline (25 micromol./kg); (d) treated with diazepam (2 mg/kg); (e) treated with diphenylhydantoin (75 mg./kg). Only rats of group (c) had seizures which lasted until the sacrifice ofthe animals. Drugs were administered intraperitoneally 25 min before the isotope (20 micro Ci of [2-3H]glycerol, which was injected into brain ventriculi), except bicuculline whose administration was simultaneous to that of labeled glycerol. Rats were sacrificed 12 min after the injection of the radioactive precursor. Cerebella were taken and lipid and water-soluble extracts examined. The treatment very much influenced the fate oflabeled, injected glycerol. Indeed, the radioactivity recovered in water-soluble extracts increased dramatically during convulsions or following the administration of any tested drugs, whereas the radioactivity incorporated into lipid decreased. The distribution of lipid radioactivity between neutral glycerolipid and glycerophospholipid was affected by seizures but not by anticonvulsive drugs. Therefore the performed treatments produced two effects: one was common to all tested drugs, whereas the other could be observed only after bicuculline.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
