Proteinograma sérico e do líquido peritoneal de equinos hígidos e daqueles submetidos à obstrução intestinal experimental
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Data
2013-11-01
Autores
Nogueira, Andressa Francisca Silva [UNESP]
Filippo, Paula Alessandra Di
Anai, Letícia Abrahão [UNESP]
Pereira, Gener Tadeu [UNESP]
Santana, Aureo Evangelista [UNESP]
Título da Revista
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Editor
Universidade Federal de Santa Maria
Resumo
Avaliou-se a resposta de fase aguda através da concentração das proteínas de fase aguda (PFA) no soro sanguíneo e no líquido peritoneal de vinte e um equinos, hígidos e submetidos à obstrução intestinal experimental, distribuídos em quatro grupos: obstrução de duodeno - GD (n=6), íleo - GI (n=6), cólon dorsal esquerdo - GM (n=6) e controle instrumentado - GC (n=3). Foram colhidas amostras de sangue e líquido peritoneal e, após centrifugação e fracionamento, as proteínas de fase aguda foram separadas por eletroforese em SDS-PAGE. Identificaram-se as proteínas IgA, ceruloplasmina, transferrina, albumina, IgG, haptoglobina, α1-glicoproteína ácida e P24, no soro e no líquido peritoneal. Houve aumento nas concentrações sérica e peritoneal de todas as PFA, sendo mais evidente no líquido peritoneal e nos animais obstruídos. O fracionamento eletroforético das PFA no líquido peritoneal é mais eficaz no diagnóstico de processos inflamatórios abdominais, quando comparado ao sérico.
It was evaluated the acute phase response over the concentration of acute phase proteins (APP) in serum and in peritoneal fluid of twenty-one healthy equines submitted to experimental intestinal obstruction distributed in groups: duodenal obstruction (GD), ileum obstruction (GI), left-dorsal colon (GM), and instrumentalized control (GC). Blood and peritoneal liquid samples were collected, after centrifugation and fractioning and the acute phase proteins were separated by electrophoresis in SDS-PAGE. The acute phase proteins (APP) identified were IgA, ceruloplasmin, transferrin, albumin, IgG, haptoglobin, α1-acid glycoprotein and P24, in the serum and liquid peritoneal. There was anincrease in the serum and peritoneal proteins concentrations evidencing a higher response of the APP from the peritoneal fluid and of the obstructed groups. The electrophoretic separation of the proteins included in the peritoneal liquid is more effective when compared to the serum concentration in the diagnosis of abdominal inflammatory processes.
It was evaluated the acute phase response over the concentration of acute phase proteins (APP) in serum and in peritoneal fluid of twenty-one healthy equines submitted to experimental intestinal obstruction distributed in groups: duodenal obstruction (GD), ileum obstruction (GI), left-dorsal colon (GM), and instrumentalized control (GC). Blood and peritoneal liquid samples were collected, after centrifugation and fractioning and the acute phase proteins were separated by electrophoresis in SDS-PAGE. The acute phase proteins (APP) identified were IgA, ceruloplasmin, transferrin, albumin, IgG, haptoglobin, α1-acid glycoprotein and P24, in the serum and liquid peritoneal. There was anincrease in the serum and peritoneal proteins concentrations evidencing a higher response of the APP from the peritoneal fluid and of the obstructed groups. The electrophoretic separation of the proteins included in the peritoneal liquid is more effective when compared to the serum concentration in the diagnosis of abdominal inflammatory processes.
Descrição
Palavras-chave
Blood, Peritoneal fluid, Horse, Colic, Acute phase proteins, Sangue, Líquido peritoneal, Equino, Cólica, Proteína de fase aguda
Como citar
Ciência Rural. Universidade Federal de Santa Maria, v. 43, n. 11, p. 2018-2024, 2013.