Publicação: Influence of salts on the coexistence curve and protein partitioning in nonionic aqueous two-phase micellar systems
Carregando...
Data
2014-12-01
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Brazilian Society of Chemical Engineering
Tipo
Artigo
Direito de acesso
Acesso aberto

Resumo
Aqueous two-phase micellar systems (ATPMS) can be exploited in separation science for the extraction/purification of desired biomolecules. Prior to phase separation the surfactant solution reaches a cloud point temperature, which is influenced by the presence of electrolytes. In this work, we provide an investigation on the cloud point behavior of the nonionic surfactant C10E4 in the presence of NaCl, Li2SO4 and KI. We also investigated the salts' influence on a model protein partitioning. NaCl and Li2SO4 promoted a depression of the cloud point. The order of salts and the concentration that decreased the cloud point was: Li2SO4 0.5 M > NaCl 0.5 M ≈ Li2SO4 0.2 M. On the other hand, 0.5 M KI dislocated the curve to higher cloud point values. For our model protein, glucose-6-phosphate dehydrogenase (G6PD), partitioning experiments with 0.5 M NaCl or 0.2 M Li2SO4 at 13.85 °C showed similar results, with KG6PD ~ 0.46. The lowest partition coefficient was obtained in the presence of 0.5 M KI (KG6PD = 0.12), with major recovery of the enzyme in the micelle-dilute phase (%Recovery = 90%). Our results show that choosing the correct salt to add to ATPMS may be useful to attain the desired partitioning conditions at more extreme temperatures. Furthermore, this system can be effective to separate a target biomolecule from fermented broth contaminants.
Descrição
Palavras-chave
Idioma
Inglês
Como citar
Brazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 31, n. 4, p. 1057-1064, 2014.