Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats

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Data

2010-01-01

Autores

Costa, V. M. [UNESP]
Langoni, Hélio [UNESP]

Título da Revista

ISSN da Revista

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Editor

Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)

Resumo

The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.

Descrição

Palavras-chave

Toxoplasma gondii, parasite in milk, female rats, PCR, bioassay

Como citar

Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 2, p. 368-374, 2010.