Avaliação da reação em cadeia da polimerase e do isolamento bacteriológico convencional na detecção de Salmonella Dublin em amostras de fezes de bezerros infectados experimentalmente
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Data
2010-06-01
Autores
Silva, D. G. [UNESP]
Silva, D. R. [UNESP]
Silva, P. R. L.
Cícero, E. A. S. [UNESP]
Ferraz, A.L.J. [UNESP]
Lemos, M. V. F. [UNESP]
Fagliari, J. J. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
Resumo
The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive samples. The SC broth was the best option as selective enrichment in both techniques.
Descrição
Palavras-chave
Calves, feces, Salmonella Dublin, PCR, culture
Como citar
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 62, n. 3, p. 752-756, 2010.