Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters
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Data
1999-01-01
Autores
Peracoli, MTS
Sugizaki, M. F.
Mendes, R. P.
Naiff, R.
Montenegro, M. R.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Kluwer Academic Publ
Resumo
Isolates of Paracoccidioides brasiliensis may vary in virulence according to time of in vitro subcultivation. The present study compared the morphology and pathogenicity to hamsters of two P. brasiliensis isolates: one obtained from human lesions and maintained in the laboratory for several years (Pb-18) and the other isolate recovered from hamsters inoculated with organ homogenates from armadillos (Pb-T). The microscopic morphology of Pb-18 and Pb-T showed yeast cells with similar diameter. However, Pb-T produced a significantly higher number of buds per mother cell than Pb-18. Besides, the mycelial form of Pb-T developed abundant sporulation during 8 weeks of culture which was absent in the Pb-18 isolate. Virulence studies demonstrated that mortality rates, antibody levels, fungal load and extent of lesions in the organs were significantly higher in animals infected with Pb-T. The results demonstrated that Pb-T recently isolated from an animal was more virulent than Pb-18. These differences between the two P. brasillensis isolates may be indicators of virulence attenuation in this fungal species.
Descrição
Palavras-chave
antibodies, armadillo, experimental infection, hamster, Paracoccidioides brasiliensis, virulence
Como citar
Mycopathologia. Dordrecht: Kluwer Academic Publ, v. 148, n. 3, p. 123-130, 1999.